| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.203566 |
| Chromosome: | chromosome 16 |
| Location: | 5782607 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g678400 | (1 of 1) PTHR22847//PTHR22847:SF412 - WD40 REPEAT PROTEIN // TRANSDUCIN/WD40 DOMAIN-CONTAINING PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAAGCCTTAGAGCGAGTAACGATGAAAT |
| Internal bar code: | GCGGACCAAAAGTGGGCGGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 781 |
| LEAP-Seq percent confirming: | 99.7812 |
| LEAP-Seq n confirming: | 3192 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTACTCGGGTGGAGAGGAC |
| Suggested primer 2: | CTCACTGCCGCTATTCACAA |