| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.203573 |
| Chromosome: | chromosome 6 |
| Location: | 5594370 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g285650 | ORC6 | (1 of 1) PF05460 - Origin recognition complex subunit 6 (ORC6) (ORC6); Origin recognition complex subunit 6 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATTGTCGCTTACTCTAGGCATAGACCAAA |
| Internal bar code: | CCCACGCCCACCGCTCGTCTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 512 |
| LEAP-Seq percent confirming: | 99.3311 |
| LEAP-Seq n confirming: | 1188 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTTGAAGAAGAGCAGGCG |
| Suggested primer 2: | GTGCAGGTCTCGTAGCACAA |