| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.203588 |
| Chromosome: | chromosome 16 |
| Location: | 4463118 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g670754 | (1 of 1) PTHR10217//PTHR10217:SF512 - VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCCTCTCTGGGCACCGGCGGCGGCGCCA |
| Internal bar code: | TGGTTCGCGATGAGAGTTGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 523 |
| LEAP-Seq percent confirming: | 99.3182 |
| LEAP-Seq n confirming: | 874 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATACCCAGTGAGGTGCTGG |
| Suggested primer 2: | ACGGTCAGTAATGTCTCCCG |