| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.203606 |
| Chromosome: | chromosome 3 |
| Location: | 1896730 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g155051 | SPA1 | Sphinganine-1-phosphate aldolase/lyase; (1 of 1) 4.1.2.27 - Sphinganine-1-phosphate aldolase / Sphingosine-1-phosphate lyase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCGCCAAGCCACAGCCGCAGCAGCCGCA |
| Internal bar code: | TACTTTCCTGTCCCTGCTCAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 309 |
| LEAP-Seq percent confirming: | 99.7042 |
| LEAP-Seq n confirming: | 13147 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGCCAGTACGGAAATGAG |
| Suggested primer 2: | GTTGGTAGGGGTTTACGGGT |