| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.203629 |
| Chromosome: | chromosome 13 |
| Location: | 1561758 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g573100 | MAM3C | (1 of 5) K16302 - metal transporter CNNM (CNNM); Protein putatively involved in mitochondrial biogenesis | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGAGCGGCTCGCCGCGGGAACAAACCCAG |
| Internal bar code: | ACCTCTACGACCGATGGAGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 968 |
| LEAP-Seq percent confirming: | 93.5791 |
| LEAP-Seq n confirming: | 23756 |
| LEAP-Seq n nonconfirming: | 1630 |
| LEAP-Seq n unique pos: | 74 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGCGTGTAGGTTTGGTGT |
| Suggested primer 2: | CTGGATCCAAAACACACACG |