| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.203659 |
| Chromosome: | chromosome 6 |
| Location: | 7247605 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g298251 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTGTTGCAGGGTGCGGCGCATAGGGTGC |
| Internal bar code: | CTCGGAGGCAAGCGCAGGGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 990 |
| LEAP-Seq percent confirming: | 99.3666 |
| LEAP-Seq n confirming: | 10354 |
| LEAP-Seq n nonconfirming: | 66 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTAGAAGAGTTGGAAGCG |
| Suggested primer 2: | CTATTCTGGGGGAGGAGGAG |