| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.203808 |
| Chromosome: | chromosome 1 |
| Location: | 1591686 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g008300 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTGTGAGGCCGGCGAGGAAAGAGCCGAG |
| Internal bar code: | CGTAGAATGACCCCGCGGCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 742 |
| LEAP-Seq percent confirming: | 52.6259 |
| LEAP-Seq n confirming: | 1453 |
| LEAP-Seq n nonconfirming: | 1308 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCACATCCATACACACACA |
| Suggested primer 2: | AGCCGTCTAGATCCGCTACA |