| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.203830 |
| Chromosome: | chromosome 7 |
| Location: | 4505180 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g343534 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTGAAAACAGCCATAATAAAGTCCAAAC |
| Internal bar code: | GTTGGTGCCGAAGCAAAAATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2 |
| LEAP-Seq percent confirming: | 96.9349 |
| LEAP-Seq n confirming: | 253 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGACACGCCATACCAGAT |
| Suggested primer 2: | TCGTGATGTTCGGTGTTTGT |