Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.203831 |
Chromosome: | chromosome 11 |
Location: | 1268489 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre11.g467705 | (1 of 3) K02154 - V-type H+-transporting ATPase subunit a (ATPeV0A, ATP6N) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGGCACGCGTAGACACACACACACACAC |
Internal bar code: | GTTCTCGGCTCGGTTACCTTCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 253 |
LEAP-Seq percent confirming: | 99.7706 |
LEAP-Seq n confirming: | 435 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTGCCCTCAAGACAGGCTAC |
Suggested primer 2: | ATCCTGTTGCGGTAGTGGAC |