| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.203855 |
| Chromosome: | chromosome 2 |
| Location: | 7953298 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g141000 | DCL1 | Dicer-like protein; (1 of 1) K11592 - endoribonuclease Dicer (DICER1, DCR1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACTCCCCATCCTCGAAAATGGCTGCTGCG |
| Internal bar code: | TTACTCGAGGTCTAGGCTAACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 341 |
| LEAP-Seq percent confirming: | 98.9858 |
| LEAP-Seq n confirming: | 488 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTGCTACCAAGAGCATGT |
| Suggested primer 2: | AACTGCCCCAAACTATGCAC |