Insertion junction: LMJ.RY0402.203871_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.203871
Chromosome:chromosome 5
Location:3145075
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre05.g239300 (1 of 85) IPR009057 - Homeodomain-like 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCAACACTGGCCACGAGGACTGTACTACT
Internal bar code:ACAGGACGGGGTCCGATATCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1029
LEAP-Seq percent confirming:99.9631
LEAP-Seq n confirming:2706
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:ACCACCTTGGAACACTTTGC
Suggested primer 2:GGACATGGCTTGTGTGATTG