| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.203889 |
| Chromosome: | chromosome 6 |
| Location: | 7464152 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g300139 | (1 of 7) IPR010995 - DNA repair Rad51/transcription factor NusA, alpha-helical | 3'UTR|intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATCCGCACGGGTACGGCCCGCAATCCCC |
| Internal bar code: | CGACGCTAGAGGGGAGTGTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1188 |
| LEAP-Seq percent confirming: | 96.7033 |
| LEAP-Seq n confirming: | 176 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGGGATATCATGGACCGT |
| Suggested primer 2: | AGCTATACAGGCCTCCAGCA |