| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.203895 |
| Chromosome: | chromosome 3 |
| Location: | 3310971 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g166250 | FAS11,FLA6 | FAS1 domain containing protein;; (1 of 1) PF00932//PF02469 - Lamin Tail Domain (LTD) // Fasciclin domain (Fasciclin) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCCACCCACCCACAGGACCGCTCCAAGC |
| Internal bar code: | CACGGACACGGTCCGCGGTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 36 |
| LEAP-Seq percent confirming: | 86.8613 |
| LEAP-Seq n confirming: | 119 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGAGGTGTGTGTGTGTGTG |
| Suggested primer 2: | TCTTCGTTGCAACACCACTC |