| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.203977 |
| Chromosome: | chromosome_11 |
| Location: | 3633148 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre11.g482200 | FBB9 | Flagellar/basal body protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATGCCGCTCCTGCTAACTTTCCTTACCACT |
| Internal bar code: | GTCAGCGTCCATACCCGGGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 562 |
| LEAP-Seq percent confirming: | 99.7856 |
| LEAP-Seq n confirming: | 931 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGAATACCAGCCCAAACT |
| Suggested primer 2: | GTTGTCCAGTTCCACACACG |