| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.204005 |
| Chromosome: | chromosome 3 |
| Location: | 3019863 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g163950 | CDO2 | (1 of 2) 1.13.11.20 - Cysteine dioxygenase; Cysteine dioxygenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGTCGTGTCGGCAGGTTCCGCGTTATCCT |
| Internal bar code: | AGGGCGTACGAGTACGGGGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 843 |
| LEAP-Seq percent confirming: | 99.5253 |
| LEAP-Seq n confirming: | 629 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGCCCAAAAAGAGCTGAT |
| Suggested primer 2: | GCCACCTCCTTGTTGTTGAT |