Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.204022 |
Chromosome: | chromosome_2 |
Location: | 2418263 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g091500 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCCAGCGACTGCGCGCCACGGGCGGCGCCC |
Internal bar code: | GTCGGTAAGTTCACCGCCATCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 819 |
LEAP-Seq percent confirming: | 97.756 |
LEAP-Seq n confirming: | 1394 |
LEAP-Seq n nonconfirming: | 32 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACCGAAACTTCAGTCCGCTA |
Suggested primer 2: | TTGCTATTCCACCAGCACAG |