Insertion junction: LMJ.RY0402.204032_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.204032
Chromosome:chromosome 11
Location:1798277
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre11.g467500 (1 of 1) PF05664 - Plant family of unknown function (DUF810) (DUF810) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGACTGGCAGTGACGGTGGGTTAGTGGGT
Internal bar code:CACGCACCTCACGCGCGGCAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:372
LEAP-Seq percent confirming:99.6639
LEAP-Seq n confirming:1186
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TCACGCAACCTCTCTCACAC
Suggested primer 2:CGTTGTGGATGTTGGTGAAG