| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.204033 |
| Chromosome: | chromosome 7 |
| Location: | 2649512 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g330550 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCGATCTGGTGGCTGTGCCATCCCTTTA |
| Internal bar code: | CTGTGAGCGCTGGAACCGTTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 140 |
| LEAP-Seq percent confirming: | 85.5972 |
| LEAP-Seq n confirming: | 731 |
| LEAP-Seq n nonconfirming: | 123 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTAGGGTCAATGTGGGGAG |
| Suggested primer 2: | CTACCAATCCCAATGCTTGC |