| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.204035 |
| Chromosome: | chromosome 6 |
| Location: | 3291615 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g277150 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCATATACTTAGTAATCAGGGGTGTGGCG |
| Internal bar code: | TCCTAAATCCGCCGAGGGTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 595 |
| LEAP-Seq percent confirming: | 97.091 |
| LEAP-Seq n confirming: | 8344 |
| LEAP-Seq n nonconfirming: | 250 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTACTAGACCACCCGCATCC |
| Suggested primer 2: | GTATGTAAAGCGCACAGGCA |