| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.204072 |
| Chromosome: | chromosome 7 |
| Location: | 6414304 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g358050 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCAGCCGCCGACTGCACTGCGCCCTGCA |
| Internal bar code: | AATGGCGGTATCTACTTTGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 537 |
| LEAP-Seq percent confirming: | 99.5464 |
| LEAP-Seq n confirming: | 6584 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGTGCGACCTAGCCAACT |
| Suggested primer 2: | GCATCCACTGGCACATACAC |