| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.204089 |
| Chromosome: | chromosome 16 |
| Location: | 2069688 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g657350 | SNRK2I | (1 of 4) PTHR24343//PTHR24343:SF167 - SERINE/THREONINE KINASE // SUBFAMILY NOT NAMED; snRK1 family in Chlamydomonas, subgroup 2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAGTCGTGCCCGCCCAGCTGGGCCCGGT |
| Internal bar code: | GCCGACATGCTCGAGGTGTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1014 |
| LEAP-Seq percent confirming: | 99.7603 |
| LEAP-Seq n confirming: | 2497 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTTGATGGTCTCGATGCTG |
| Suggested primer 2: | GGCAGAGGAACATGGGTAGA |