| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.204117 |
| Chromosome: | chromosome_2 |
| Location: | 563972 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g077600 | FAP51 | Flagellar Associated Protein | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTGATTCAACCGGGGGGAAATGAGAGCTGC |
| Internal bar code: | GCATCGGTTTCGGACTGGTTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 153 |
| LEAP-Seq percent confirming: | 10.6256 |
| LEAP-Seq n confirming: | 107 |
| LEAP-Seq n nonconfirming: | 900 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGGGATATCATGGACCGT |
| Suggested primer 2: | GGAGACACCATTAGGGGGAT |