| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.204136 |
| Chromosome: | chromosome_17 |
| Location: | 5596903 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g737850 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AATACGGTACATCACCTCGTACCCTAATAC |
| Internal bar code: | AGGTAGTCGGTGAGCTATGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 947 |
| LEAP-Seq percent confirming: | 99.0099 |
| LEAP-Seq n confirming: | 100 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCGGATCAATTTGCATAG |
| Suggested primer 2: | GTGCTCACGTATGCAGGTGT |