| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.204156 |
| Chromosome: | chromosome_1 |
| Location: | 5004662 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g034550 | FAP109 | Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGTTGTTTGTGTTCGTCAGTGCTCCAGACA |
| Internal bar code: | TCGAGGGAGCGTCAGTTCAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 961 |
| LEAP-Seq percent confirming: | 99.5588 |
| LEAP-Seq n confirming: | 3385 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGCGACCTCCTAAGCAAT |
| Suggested primer 2: | CAGCATCTTCACGGACTTCA |