| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.204161 |
| Chromosome: | chromosome 14 |
| Location: | 2206440 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g622800 | (1 of 1) IPR000104//IPR002921//IPR029058 - Antifreeze protein, type I // Fungal lipase-like domain // Alpha/Beta hydrolase fold | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGACTTTCGCAGATGGCCGGTTCTCGCAG |
| Internal bar code: | GCGCGTACTTAACGAGTCTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 214 |
| LEAP-Seq percent confirming: | 99.6964 |
| LEAP-Seq n confirming: | 2299 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCTGCTGATACTGCAAG |
| Suggested primer 2: | CTTTACGGCCCGATAACTCA |