| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.204164 |
| Chromosome: | chromosome 12 |
| Location: | 3223135 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g498750 | LIP2,LIPG2 | (1 of 1) K14452 - gastric triacylglycerol lipase (LIPF); Putative triacylglycerol lipase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGCGCACTGGGCGCAGGCGCGTGTCTAT |
| Internal bar code: | TAGCCCCGCTGGGGCCACCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1460 |
| LEAP-Seq percent confirming: | 99.1321 |
| LEAP-Seq n confirming: | 5140 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTCGTTGAGTGGGATGTG |
| Suggested primer 2: | GGAGGGCACACTTAGCAGAG |