| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.204223 |
| Chromosome: | chromosome 6 |
| Location: | 3523694 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278109 | FAD2B | Putative Oleate/fatty acid desaturase, _-12; (1 of 1) 1.14.15.3 - Alkane 1-monooxygenase / Omega-hydroxylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGAGTAGGGTGCAAATCGGAGGTGTGGG |
| Internal bar code: | GTTCATGCCGAATCGAACAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 223 |
| LEAP-Seq percent confirming: | 99.6944 |
| LEAP-Seq n confirming: | 3262 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGTGTCGCACATCCATAC |
| Suggested primer 2: | CTGCTGAGTGTCACCGTGTT |