| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.204249 |
| Chromosome: | chromosome 6 |
| Location: | 3779596 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278161 | (1 of 40) 3.4.24.38 - Gametolysin / Lysin | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGAAGTTGTAAGAGGGCGTGGCCCAGCCG |
| Internal bar code: | ACGGGGCTTTGCGAAGCAGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 222 |
| LEAP-Seq percent confirming: | 95.6757 |
| LEAP-Seq n confirming: | 1062 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTAGGTGGCGTTGTAGGAA |
| Suggested primer 2: | ATCATCGCAGAATCTGGGTC |