| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.204265 |
| Chromosome: | chromosome 12 |
| Location: | 9239818 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g541600 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGCCAGCCGCCCGCTCCCCCAAGCAAGG |
| Internal bar code: | TGCTGGTATTTGGTGCCGATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 445 |
| LEAP-Seq percent confirming: | 99.1981 |
| LEAP-Seq n confirming: | 2969 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGTGCAGGATGCAGTAAA |
| Suggested primer 2: | CCCTGTTGTCATGATTGCAC |