| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.204285 |
| Chromosome: | chromosome 4 |
| Location: | 2741444 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g223550 | (1 of 26) IPR011990 - Tetratricopeptide-like helical domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAGTCACATGCACAAAAGACATAAGGCAC |
| Internal bar code: | GCAGGATTTGAAGCGTTGGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 809 |
| LEAP-Seq percent confirming: | 80.8068 |
| LEAP-Seq n confirming: | 661 |
| LEAP-Seq n nonconfirming: | 157 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGATTGAGCAGGAGTGAT |
| Suggested primer 2: | TTCACTGCCATCAAGTCGAG |