| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.204307 |
| Chromosome: | chromosome 12 |
| Location: | 2582037 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g505250 | FAP288 | (1 of 3) PF13202//PF13499 - EF hand (EF-hand_5) // EF-hand domain pair (EF-hand_7); EF Hand Containing Flagellar Associated Protein 288 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCGCGCCCGCTTTCGTTTGAGCGCCCC |
| Internal bar code: | GTCGAGTGATAAAAACAAGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 778 |
| LEAP-Seq percent confirming: | 99.4413 |
| LEAP-Seq n confirming: | 712 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTCTGTACACCCGAAAGG |
| Suggested primer 2: | AGGTTGGAGAACGGGATCTT |