| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.204327 |
| Chromosome: | chromosome 2 |
| Location: | 1750181 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g086456 | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGGCTTGGTAGTCACGTTCGTCTCACGA |
| Internal bar code: | CGGACGCCCAGGACGGGGGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 811 |
| LEAP-Seq percent confirming: | 99.4753 |
| LEAP-Seq n confirming: | 3223 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCGATTAATGCGTAGGGA |
| Suggested primer 2: | ACAAACAACAAGGAGGTGGC |