Insertion junction: LMJ.RY0402.204339_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.204339
Chromosome:chromosome 6
Location:3441360
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g278092 (1 of 41) PF13920 - Zinc finger, C3HC4 type (RING finger) (zf-C3HC4_3) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGGCCCCCACATTCATTGCCGCCATCAC
Internal bar code:TCTGGATTCTGAGTACGCACCC

Confirmation - LEAP-Seq

LEAP-Seq distance:923
LEAP-Seq percent confirming:84.0506
LEAP-Seq n confirming:664
LEAP-Seq n nonconfirming:126
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:GCACAAGGCTCTGTACACCA
Suggested primer 2:CAGTGCATGGAAACATACCG