| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.204364 |
| Chromosome: | chromosome 6 |
| Location: | 5738435 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g287100 | CAM11 | Calmodulin-like protein; (1 of 2) PF13499//PF13833 - EF-hand domain pair (EF-hand_7) // EF-hand domain pair (EF-hand_8) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGCTCCATTCCATCTCGCTCATGTGTGC |
| Internal bar code: | TTTGTATCAGGGCGCGGCAACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 389 |
| LEAP-Seq percent confirming: | 99.8074 |
| LEAP-Seq n confirming: | 4145 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGCATGGAACACATTGTC |
| Suggested primer 2: | CAGTAGCAGTAGTGCAGGCG |