| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.204366 |
| Chromosome: | chromosome_11 |
| Location: | 3669538 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre11.g482483 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GACAGCGGTGCAGCAACGAGGCCCGCCCAC |
| Internal bar code: | ATCGTTGATCGTTTGTGCTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 148 |
| LEAP-Seq percent confirming: | 98.9933 |
| LEAP-Seq n confirming: | 295 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACCTCTGACTTCTCCTGC |
| Suggested primer 2: | ACACACACCCACACACAACC |