| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.204370 |
| Chromosome: | chromosome 10 |
| Location: | 173461 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g418950 | KIN10-2,KIN10B,KIN4-4,IAR1 | Kinesin motor protein; (1 of 2) PTHR24115:SF136 - KLP31E, ISOFORM A | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCGTGTTGCGCGTGGTCCATCCCTCACC |
| Internal bar code: | GTGCTATCGTTGGCCAGGCTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 958 |
| LEAP-Seq percent confirming: | 99.2014 |
| LEAP-Seq n confirming: | 2236 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAAGTCCTTACGTTGCAG |
| Suggested primer 2: | TTGCATGCAGCTGTCACATA |