| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.204392 |
| Chromosome: | chromosome 12 |
| Location: | 3706101 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g514600 | (1 of 1) 2.7.4.27 - ([Pyruvate, phosphate dikinase] phosphate) phosphotransferase / Pyruvate, phosphate dikinase regulatory protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACACGGGAATGACGCCGGTCTGGGAGAGG |
| Internal bar code: | TGATCATAGCCAGTTAGTAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 484 |
| LEAP-Seq percent confirming: | 97.5 |
| LEAP-Seq n confirming: | 585 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAATGGGTAGCGTGTGGTC |
| Suggested primer 2: | CTGCGTATTTCTTCCCGTGT |