| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.204394 |
| Chromosome: | chromosome 16 |
| Location: | 275138 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g694250 | (1 of 18) PF12937 - F-box-like (F-box-like) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTGTACGTGGTGCGGTGCGACGCTGTCG |
| Internal bar code: | AGGATGGGACGATGGGATAAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 189 |
| LEAP-Seq percent confirming: | 99.4837 |
| LEAP-Seq n confirming: | 578 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAAATCCAGTCTGTCGGTT |
| Suggested primer 2: | AACGGCTTAGTACCGTGTCG |