Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.204416 |
Chromosome: | chromosome 8 |
Location: | 1294647 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre08.g363700 | (1 of 1) K18647 - acidic leucine-rich nuclear phosphoprotein 32 family member B (ANP32B) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTGCATGCGCCATGAGGGGCTTAGGCAA |
Internal bar code: | CGGTCAAATAGGATATCGGCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 462 |
LEAP-Seq percent confirming: | 99.4769 |
LEAP-Seq n confirming: | 4754 |
LEAP-Seq n nonconfirming: | 25 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTCGAAGTCCTCCTCCTCCT |
Suggested primer 2: | TTATTGCTCAGTGTGGCGAG |