| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.204433 |
| Chromosome: | chromosome 12 |
| Location: | 5754353 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g533050 | (1 of 1) K14538 - nuclear GTP-binding protein (NUG1, GNL3) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCATCCTAATCACTAACCCAGCAACCTG |
| Internal bar code: | AAACGTTTGGGAGGTACAATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 360 |
| LEAP-Seq percent confirming: | 99.7145 |
| LEAP-Seq n confirming: | 3143 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCGTGGCAGTAACAACTC |
| Suggested primer 2: | CTCAAAAAGGCACGGCTAAG |