| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.204437 |
| Chromosome: | chromosome 9 |
| Location: | 6026516 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g403108 | (1 of 1) K16601 - tubulin polyglutamylase TTLL4 [EC:6.-.-.-] (TTLL4) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACGTTCATATGTTGTCGGCGATGTTGAA |
| Internal bar code: | GGTCAAAGCAGGGGTAGGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 680 |
| LEAP-Seq percent confirming: | 95.3184 |
| LEAP-Seq n confirming: | 14700 |
| LEAP-Seq n nonconfirming: | 722 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATAACACGTGCACATCCCG |
| Suggested primer 2: | AGACAAAAATGGCTGGATGG |