| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.204466 |
| Chromosome: | chromosome 1 |
| Location: | 2989229 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g018350 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTGTGCGCCGCGTCACCATCACCTGTGG |
| Internal bar code: | AGTCGGGGACCAAATAAGTTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 731 |
| LEAP-Seq percent confirming: | 99.7283 |
| LEAP-Seq n confirming: | 11746 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGCTGACCTCAAACGACA |
| Suggested primer 2: | CAATTTTGTTCGTGTGACCG |