| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.204547 |
| Chromosome: | chromosome 3 |
| Location: | 5901725 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g190000 | PPP14 | Phosphoprotein phosphatase 2C-related; (1 of 70) 3.1.3.16 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTCAAAGGTAAACACGTCCGGGTCGCAC |
| Internal bar code: | TCCAGCTCTGGCCCCCGAGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 570 |
| LEAP-Seq percent confirming: | 93.5484 |
| LEAP-Seq n confirming: | 435 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACAACAGACATGGAACCG |
| Suggested primer 2: | CTGGAGGAGGAGAGCGAGT |