| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.204549 |
| Chromosome: | chromosome 4 |
| Location: | 3698855 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g229100 | (1 of 2) 2.7.1.158 - Inositol-pentakisphosphate 2-kinase / IP5 2-kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGTTGGCAGCCCCGGCGCCTTTGAGGCT |
| Internal bar code: | GATGGAGCGTGATTACTGGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 699 |
| LEAP-Seq percent confirming: | 99.3998 |
| LEAP-Seq n confirming: | 4637 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTCTTTCCCTGGATTCCC |
| Suggested primer 2: | GCTGTCTGGCGAGTCCTAAC |