| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.204555 |
| Chromosome: | chromosome 1 |
| Location: | 2032427 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g010880 | (1 of 2) K18598 - echinoderm microtubule-associated protein-like 6 (EML6) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGATGGGGGGACGTGCTCAATAGGGAAGC |
| Internal bar code: | GCGCCTCGACCGGAGCCCCTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 25 |
| LEAP-Seq percent confirming: | 99.7347 |
| LEAP-Seq n confirming: | 376 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGTGTCGTGCAAGCAGTA |
| Suggested primer 2: | CCTTGACTCTTGGCACCTTC |