| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.204558 |
| Chromosome: | chromosome 11 |
| Location: | 1268913 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467705 | (1 of 3) K02154 - V-type H+-transporting ATPase subunit a (ATPeV0A, ATP6N) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTGAATCAGCAGAAGTAGAACAAAAGGC |
| Internal bar code: | CGGAGTCCTTACGACCGGTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 715 |
| LEAP-Seq percent confirming: | 99.6189 |
| LEAP-Seq n confirming: | 4182 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCTGTTGCGGTAGTGGAC |
| Suggested primer 2: | CTGCCCTCAAGACAGGCTAC |