| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.204620 |
| Chromosome: | chromosome 17 |
| Location: | 3732318 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g727400 | HRP8 | Hydroxyproline-rich glycoprotein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTCACACGCGCTATACAACCCGTGCTTT |
| Internal bar code: | GCCCGTGTGCCCGACAGAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 398 |
| LEAP-Seq percent confirming: | 91.6208 |
| LEAP-Seq n confirming: | 4658 |
| LEAP-Seq n nonconfirming: | 426 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTTTCACAGCCCCTGACAC |
| Suggested primer 2: | CCACCATGTCACAGTTTTGC |