Insertion junction: LMJ.RY0402.204631_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.204631
Chromosome:chromosome 7
Location:2367307
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g328350 FAP207 Flagellar Associated Protein 207; (1 of 14) PF02493 - MORN repeat (MORN) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAAACCTCTATGTTTCTCAAATGACCTCGT
Internal bar code:GGCAACTTCTGACGGGAGGGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:676
LEAP-Seq percent confirming:93.0406
LEAP-Seq n confirming:1992
LEAP-Seq n nonconfirming:149
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TCGATGTTGCTGTTTCTTGC
Suggested primer 2:AGATAGTGGTATGCCCACGC