| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.204631 |
| Chromosome: | chromosome_7 |
| Location: | 2367307 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g328350 | FAP207 | Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CAAACCTCTATGTTTCTCAAATGACCTCGT |
| Internal bar code: | GGCAACTTCTGACGGGAGGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 676 |
| LEAP-Seq percent confirming: | 93.0406 |
| LEAP-Seq n confirming: | 1992 |
| LEAP-Seq n nonconfirming: | 149 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGATGTTGCTGTTTCTTGC |
| Suggested primer 2: | AGATAGTGGTATGCCCACGC |