Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.204646 |
Chromosome: | chromosome_3 |
Location: | 3486902 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g167600 | FAP61 | Flagellar Associated Protein | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGAACAGCCTGGCGCCCAAGTACCAGGCCG |
Internal bar code: | CTTGCCATGCATGGTGCGACAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 936 |
LEAP-Seq percent confirming: | 99.1849 |
LEAP-Seq n confirming: | 2677 |
LEAP-Seq n nonconfirming: | 22 |
LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGGTTTGGTTTAGTTTCGGC |
Suggested primer 2: | ACCAAACCCACTCGAACAAG |