Insertion junction: LMJ.RY0402.204683_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.204683
Chromosome:chromosome 6
Location:3767002
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g278158 (1 of 2) IPR001283//IPR003882//IPR014044 - Cysteine-rich secretory protein, allergen V5/Tpx-1-related // Pistil-specific extensin-like protein // CAP domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCTCACCCGCGACACGCTCACACACCCAC
Internal bar code:AAGCGTCACGCAGTAAAGCGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:391
LEAP-Seq percent confirming:99.8025
LEAP-Seq n confirming:1516
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GGCTTGAGCCCATTATTGAA
Suggested primer 2:CGTTCGATAGGTCGGTTGTT